Agents Which Mediate Pulmonary Edema
Abstract
The work described in this report has focused on delineating various approaches to understanding mechanisms of pathological changes leading to pulmonary edema. Baseline parameters were determined for the sheep lung fistula model. Bronchoalveolar lavage (BAL) was performed on 100 sheep and alveolar macrophages (AM) obtained by this method were used for 7 functional baseline studies. Isolated rat lung was used for perfusion studies. Isolated sheep lung perfusion studies were unsuccessful; instead isolated rat lung was used for perfusion studies. Permeability of the alveolar capillary membrane in sheep was assessed by analysis of protein in BAL. Analysis of arachidonate metabolites in BAL by high performance liquid chromatography is under progress, and results on leukotriene B4 levels are presented. Finally studies on pulmonary endothelium have proceeded in four areas: (1) establishment of cell lines, (2) assessment of cytotoxicity by lymph, (3) adherence of neutrophils, and (4) permeability of monolayers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1990
- Accession Number
- ADA246029
Entities
People
- Frederick Glauser
Organizations
- Virginia Commonwealth University School of Medicine