IGG Subclass and Isotype Specific Immunoglobulin Responses to Lassa Fever and Venezuelan Equine Encephalomyelitis: Natural Infection and Immunization
Abstract
Venezuelan Equine Encephalitis(VEE) specific immunoglobulin responses to the two vaccines, TC-83(A live-attenuated vaccine) and C-84(A formulin inactivated vaccine derived from the TC-83 strain) were evaluated using an antigen and isotype specific enzyme-linked immunoabsorbent assay(ELISA). The VEE-specific ELISA was developed and standardized using selected sera from individuals previously immunized. Initial experiments demonstrated that non- specific binding in the IgG, G2, A and M analysis was a significant problem in data interpretation. Preparation of a negative control(tissue culture media derived) antigen were utilized to further standardize assays. All assays were standardized in methodology and in relation to a uniform reference curve, where available. Initial screening of 28 SERA demonstrated the presence of predominantly VEE-specific Gl and G3 subclasses, IgA and M response were also demonstrated in the series of subjects who received the TC-83 vaccine as well as in response to a C-84 boost vaccine.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 30, 1991
- Accession Number
- ADA246912
Entities
People
- Renata J. Engler
Organizations
- Uniformed Services University of the Health Sciences