Role of Protein Phosphorylation in the Regulation of Neuronal Sensitivity
Abstract
The project reported here focuses on the regulation of neuronal sensitivity by a novel class of protein kinase: an ecto-protein kinase which phosphorylates proteins at the cell surface by extracellular ATP. We proposed that the ecto-protein kinase that our previous studies have described in neuronal cells may play a significant role in the regulation of neuritogenesis and synaptogenesis. Our studies are designed to provide experimental evidence in support of this hypothesis. The progress we have made, in this project includes the development of novel experimental paradigms for the determination of ecto-protein kinase and its substrates in cultured cells. We used these paradigms in the conclusive identification of the surface phosphoproteins in primary neurons cultured from embryonic brain and in PC12 cloned neuronal cells induced to differentiate by nerve growth factor (NGF). We have determined which of these surface phosphoproteins are regulated by NGF.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 06, 1992
- Accession Number
- ADA246950
Entities
People
- Yigal H. Ehrlich
Organizations
- City University of New York