The Molecular Anatomy of PFDA Hepatotoxicity as Studied by Two-Dimensional Electrophoresis
Abstract
Perfluoro-n-decanoic acid (PFDA) effects on protein expression in the rat liver were studied in rodents following in vivo exposure to PFDA levels above, below and at the LD-50. Two-dimensional whole liver homogenate protein patterns were generated and compared to previous results. As before, numerous proteins were altered; some suppressed, some induced, but most were unaffected. In an effort to identify the altered proteins, further analysis of basic proteins by first-dimension NEPHGE revealed the induction of cytochrome P452 (lauric acid omega-oxidase) and enoyl-CoA hydratase. Induction of these and related enzymes confirms previously observed PFDA-induced peroxisome proliferation and lends strong support to the notion that PFDA blocks normal Beta-oxidation, causes fatty acid accumulation, and results in compensatory peroxisomal and mitochondrial omega-and Beta oxidation continued identification of other altered proteins will be undertaken to add to the metabolic paths affected by PFDA to further delineate its toxic mechanism.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 14, 1992
- Accession Number
- ADA246952
Entities
People
- Frank A. Witzmann