Stain Test Modules for Periodontal Diagnosis
Abstract
Studies in our laboratories and clinics since the beginning of this contract with direct cytochemical methods for the light and electron microscopic demonstration and discrimination of neutrophils , B-lymphocytes and plasma cells and T-helper and suppressor cells have shown the utility of these methods in demonstrating active periodontal disease. A variation of the periodic acid- Schiff(PAS) method known as the PATS reaction has proven very important in positively staining the types of Gram negative bacteria present in subgingival plaques. At the end of last year it was noted that activated macrophages could be demonstrated in smears of subgingival plaque or crevicular fluid from periodontal disease sites by out PATS reaction as well as in gingival biopsy specimens as had previously been reported by others. The fact that these cells were macrophages, not neutrophils, was supported by their greater acid phosphatase, nonspecific esterase and proteolytic enzyme activities as well as their size, morphology and increased numbers at diseased sites shown by cytochemical methods for light and electron microscopy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 18, 1989
- Accession Number
- ADA247285
Entities
People
- Jacob S. Hanker
Organizations
- University of North Carolina at Chapel Hill