Triannual Report, January 1992, Contract N00014-90-C-0195 (Vestar, Inc.)
Abstract
A summary of relevant experiments is presented below: Dilution and ionic strength effects: LEH (Liposome Encapsulated Hemoglobin) was diluted with phosphate saline buffer or phosphate sucrose buffer to evaluate the effect of dilution and ionic strength on aggregation of the liposomes. Saline buffer had no noticeable effect as measured by mean size. However sucrose buffers seemed to cause some LEH disaggregation. LEH was produced using bovine Hb in 30 mM phosphate with 250 mM saline buffer at pH 7.4 and 5 mM phosphate with 263 mM sucrose buffer at both pH 7.4 and pH 8.3. Effects of pH and ionic strength were evaluated along with Hb recovery and in-process losses. Little effect was seen on mean size. However blood characteristics such as P50 were improved at pH 7.4, and the Hb recovery was 22% with sucrose vs. 12.6% with saline. Dialysis of LEH against 9% sucrose, 5mM lactobionate with 9% sucrose, 30 mM phosphate with 9% sucrose showed that phosphate sucrose buffer gave the best size results.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 02, 1992
- Accession Number
- ADA247544