Polymerase Chain Reaction Based Diagnostic Assays for Pneumocystis Carinii.

Abstract

The objectives of this study are to develop polymerase chain reaction assays for the-identification and quantitation of Pneumocystis carinii infections in the lungs, solid organs, bronchioloalveolar lavage fluids and blood of rats and humans. Work during the initial project period consisted of implementing qualitative reaction capable of identifying the organism in humans and rats, developing the quantitation necessary for using the assay in rats, investigation the efficacy of the assay in following the development and treatment of Pneumocystis carinii infections in the lungs and BAL fluids of rats, and attempting to implement the assay in a sensitive and reliable way in blood. with the exception of implementing the assay in a sensitive and reliable manner in blood, all the objectives for the first project period have been met. Diagnosis, HIV, RA I, PCR, P. Carinii, Lab Animals, Rats.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1992
Accession Number
ADA248259

Entities

People

  • Cynthia Wright
  • Mark Tsai
  • Melanie Cushion
  • Thomas Fanning
  • Timothy J. O'leary

Organizations

  • Armed Forces Institute of Pathology

Tags

DTIC Thesaurus Topics

  • Animals
  • Biomedical Research
  • Bone Marrow
  • Chain Reactions
  • Chemical Reactions
  • Counting Methods
  • Drug Abuse
  • Experimental Data
  • Experimental Design
  • Hematologic Tests
  • Infection
  • Laboratory Animals
  • Pcr Testing
  • Pneumocystis Carinii
  • Polymerase Chain Reaction
  • Tissues
  • Wound Infections

Fields of Study

  • Medicine

Readers

  • Cardiovascular Physiology
  • Infectious Disease/Epidemiology
  • Systems Analysis and Design