The Effect of Hyperbaric Oxygen and Pentoxifylline on the Rate of Neovascularization in Mice
Abstract
A polyvinyl alcohol sponge was implanted in mouse subcutaneous tissue to investigate two treatments (intermittent hyperoxia (100% oxygen for 90 mins twice a day at 250 kPa) and epidermal growth factor (EGF) which may modulate fibroblast infiltration. Two conditions were established for treatment: of animals to chronic hypoxia (12% oxygen for 23 hr/day), simulating low oxygen tensions in problem wounds, and normoxia (2 1% oxygen). In experiments evaluating EGF, sponges were implanted whose core contained EGF covered with a slow release polymer, the other group with placebo. Sponges were harvested at 15, 25, or 32 days after implantation. The area of the disc infiltrated by fibroblasts was measured by planimetry. After 32 days exposure to hypoxic conditions (7 days before sponge implantation and 25 days after) EGF slightly increased (NS) the area of fibroblast infiltration to placebo both hypoxic and normoxic conditions. No significant differences were observed be the hypoxically conditioned and normoxic controls. Neither chronic hypoxia alone nor chronic hypoxia with intermittent hyperbaric oxygen a 21-32 days after disc implantation affected the area of fibroblast infiltration. EGF significantly increased the area of the fibrous capsule around small PVA sponges after 15 days under normoxic conditions. Fibroblast-Hyperbaric, Oxygen-Hypoxia-Polyvinyl alcohol sponge-wound.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 27, 1992
- Accession Number
- ADA248673
Entities
People
- D. W. Criswell
- W. J. Mehm
Organizations
- Armed Forces Institute of Pathology