Translational Regulation of Cloned Genes
Abstract
The actors controlling translational efficiency of mRNA in yeast were studied to aid in design of more efficient vectors for producing heterologous proteins in yeast. A plasmid was constructed with heterologous gene production driven by the yeast CUPI promoter and the killer virus M segment translation initiation signal. Although capped RNAs were more efficiently translated in vitro than uncapped RNAs of the same sequence, changes in the 5' untranslated leader rendered uncapped RNA as translatable as wild-type capped RNA. This result may make cytoplasmic RNA-based cloning vectors feasible in yeast. Yeast, Recombinant DNA gene expression, Translation, Transcription, Polymerase chain reaction, mRNA Cap.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 04, 1992
- Accession Number
- ADA248892
Entities
People
- Michael J. Leibowitz
Organizations
- United States Army Research Laboratory