Evaluation of Dried Storage of Platelets and RBC for Transfusion Lyophilization and Other Dehydration Techniques.
Abstract
To explore and develop protocols for drying blood platelet preparations for extended storage without undue compromise of hemostatic function or cell integrity upon rehydration. A successful project outcome would greatly facilitate the use of platelets in the medical support of combat casualties with severe blood loss, major surgical repair, or extensive burn injury. The three collaborating performance sites are bringing together expertise in cell stabilization techniques and platelet function testing both in vitro and in vivo. The ultimate goal is to identify a practical procedure to produce a stabilized platelet preparation that can be stored virtually indefinitely while maintaining transfusion efficacy. Such a product would also be useful in civilian blood banking. The third year of this grant saw advancement of the animal model studies testing the hemostatic capabilities and circulatory survival of dried platelets stabilized with paraformaldehyde, refinement of an alternative platelet stabilization method using permanganate and trehalose, and an initial study of dried RBC in an in vivo circulatory model. In normal canine experimental subjects, rehydrated canine platelets were found in circulation for at least 24 hours after infusion; similar results were obtained in bleeder pigs (a congenital strain with von Willebrand's disease). Rehydrated platelets were shown to adhere to subendothelium in situ when the animal subjects were exposed to a vessel stenosis and injury protocol or subjected to a bleeding time test.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 21, 1992
- Accession Number
- ADA249914
Entities
People
- Arthur P. Bode
- Marjorie S. Reed
- Robert L. Reddick
- Stein Holme
- W. A. Heaton
Organizations
- East Carolina University