The Design of Oligonucleotides Which Attack Specific Gene Targets.

Abstract

Triple Helix Design Principles. The first research priority of the program of Navy support was to refine our understanding of triple helix forming oligonucleotides (TFOs). Binding affinity and strand orientation of triplex forming oligonucleotides were measured as a function of base composition. Based upon that work, which was published in Biochemistry (ref. 3), we showed that triple helices containing GGC and TAT triplets were stable at physiological pH and prefer to bind with an antiparallel strand orientation. This study and the accompanying patent application provided the first evidence that TFOs can bind in a site selective fashion at physiological pH and the first explicit evidence for a new (antiparallel) class of triple helix. This work also served as the basis for the filing of a continuation in part to a patent application, filed 12/89. DNA Recognition, Triplet helix formation.

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Document Details

Document Type
Technical Report
Publication Date
May 18, 1992
Accession Number
ADA250783

Entities

People

  • Michael E. Hogan

Organizations

  • Baylor College of Medicine

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Abstracts
  • Acids
  • Biochemistry
  • Biological Sciences
  • Cells
  • Chemical Compounds
  • Chemistry
  • Cultured Cells
  • Gene Expression
  • Military Research
  • Molecular Biology
  • Molecules
  • Nucleic Acids
  • Orientation (Direction)
  • Patent Applications
  • Patents
  • Recognition

Fields of Study

  • Chemistry

Readers

  • Molecular Genetics
  • Systems Analysis and Design
  • Technical Research and Report Writing.