Pathogenesis of DIC in Strain 13 Guinea Pigs Infected with Pichinde Virus.
Abstract
Pichinde virus infection of inbred guinea pigs is a model for arenaviral infections in humans. Infected animals experience reduced activity of multiple coagulation factors caused be either DIC or impaired factor synthesis due to liver injury. An RIA of guinea pig fibrinopeptide A (gFPA) has been developed to measure the degree of thrombin action in vivo. gFPA was synthesized via the solid-phase method and conjugated to BSA. High titer avid antisera were prepared in rabbit against gFPA-BSA conjugate. A double antibody RIA was establised employing goat anti-rabbit IgG to precipitate the primary complex composed of I-12-Tyr-gFPA and rabbit anti-gFPA-BSA. The working range for gFPA was between 1 ng/m1 and 1 ug/m1. The immunologically cross-reactive material, i. e., fibrinogen and FDP, was removed by mixing the plasma with ethanol (ratio 1:3) and centrifuging the precipitate. Plasma gFPA immunoreactivities of outbred guinea pigs averaged 6.56 nq/m1.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 31, 1992
- Accession Number
- ADA250972
Entities
People
- James P. Chen
Organizations
- University of Tennessee Medical Center