Development of an RNA Assay to Access HIV-1 Latency.
Abstract
HIV-1 is the cause of a slowly progressive immunosuppressive disorder, characterized by a prolonged latent phase with low levels of virus replication, and a subsequent symptomatic phase with higher levels of virus replication. Viral regulatory factors such as the virion infectivity factor (VIF), the traps-activator protein (TAT), the regulator of virus production (REV), of the negative factor (NEF) may play a role determining the level of virus replication in vivo, and thus the progression to symptomatic disease. The current proposal is proposal continuation of DAMD contract for developing a diagnostic test with prognostic capabilities. The previous study was successful in the development of a sensitive, quantitative assay for HIV-1 DNS sequences. The current proposal is for the development of a complementary assay to hypothesis to examine specific HIV-1 RNA transcripts which may provide additional diagnostic utility. One hypothesis to be tested is that the presence or absence of mRNAs for VIF, TAT/REV, and/or NEF will be closely associated with the stage of clinical disease. Alternatively, the levels of these mRNAs or their predicted protein sequences will correlate closely with the stage of disease. The RA 1; PCR amplification; RNA assay; HIV latency.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 19, 1991
- Accession Number
- ADA251728
Entities
People
- Lee Ratner
Organizations
- University of Washington