Catalytic Antibodies.
Abstract
To induce catalytic antibodies that promote redox reactions through the use of haptens that feature: (1) a nicotinamide cofactor and (2) a mono- or binuclear copper complex. A3, 4-disubstituted pyridine was synthesized (last Progress Report), conjugated to a carrier protein, and 20-monoclonal antibodies were isolated. These were tested for their ability to catalyze either a formate dehydrogenese reaction or the addition of cyanide anion to the pyridinium ring. No catalysis was found. Fluorescence titrations of the antibodies with the nicotinamide hapten both in the oxidized and reduced forms demonstrated binding affinities for both oxidation states. We concluded that this set of antibodies was not recognizing the heterocyclic portion of the hapten, therefore we discontinued studying them.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 14, 1992
- Accession Number
- ADA252019
Entities
People
- Stephen J. Benkovic
Organizations
- Pennsylvania State University