In Vitro System for Studying Metabolism of Environmental Chemicals in Human Cells.
Abstract
The objective of the project is to establish and use an in vitro system of intact hepatocytes from rodent and human liver to develop quantitative data on the metabolism of toxic chemicals that can be used in risk assessments. The following halogenated aliphatic solvents are being studied: chloroform, 1,1, 1-trichloroethane, trichloroethylene, dichloromethane, bromochloromethane, and carbon tetrachloride. During the first year of the project, experiments were performed with hepatocytes isolated from male F344 rats and the precision-mt liver slice method was established for use with rat and human liver. In the hepatocyte experiments, the cells were incubated in gastight flasks with chloroform to establish a model for measuring the rate of its disappearance. Using low concentrations of chloroform (2400 pmol per flask and 9350 pmol per flask), it was found that chloroform in the headspace of flasks containing cells rapidly decreased while it remained unchanged in control flasks. Chloroform levels in the medium also decreased with time. Future experiments will more completely characterize the kinetics of chloroform disappearance with isolated hepatocytes and compare them to precision-cut liver slices.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 29, 1992
- Accession Number
- ADA252078
Entities
People
- Carol E. Green
Organizations
- SRI International