Voltammetric Measurement of Oxygen in Single Neurons Using Platinized Carbon Ring Electrodes.
Abstract
Nafion-coated ultrasmall platinum ring electrodes have been implanted in the giant dopamine neuron of the pond snail, Planorbis corneus and the oxygen concentration inside these single neurons has been estimated. Experimental data suggest that the intracellular oxygen level in the identified dopamine neuron of Planorbis corneus is approximately 0.032 mM. 'Me oxygen concentration immediately outside the cell (approx. 10 micro m away from the cell) is 0.041 MM. Furthermore, staircase voltammetry can be used to monitor dynamic changes in oxygen concentration inside cell after bathing in Ringer's solution saturated with air/oxygen. Data obtained for intracellular oxygen concentrations suggest that intracellular oxygen consumption is increased following potassium chloride- induced stimulation of these cells. Nafion-coated platinum electrodes, dynamic changes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 10, 1992
- Accession Number
- ADA252191
Entities
People
- Andrew G Ewing
- Takayuki Abe
- Yau Y. Lau
Organizations
- Pennsylvania State University