Immunologic Intervention in HIV Infection: Anti-Polymerase Responses and Hormonal Regulation.
Abstract
10-mers using a single amino acid overlap have been prepared from the three immunogenic domains of HIV-1 Pol which correlate best with reverse transcriptase activity (amino acids 144-191, 214-335, 511-536). These 205 overlapping decapeptides were incorporated onto pins using technology developed by Cambridge Research Biochemicals, and utilized in ELISA assays with serum samples from HIV+ individuals at various clinical stages of HIV disease. Several discrete epitopes have been identified which correlate not only with capacity to block the catalytic activity of HIV RT, but also correspond to regions identified as inhibitory using murine anti-Pol monoclonal antibodies. RAI; AIDS; Rodents; Immunotherapy; Viral Activators; Cellular Immunity; Trans-Activation.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1992
- Accession Number
- ADA253464
Entities
People
- Jeffrey Laurence
Organizations
- Cornell University