Identification of Specific Protein Markers that Correlate with Initiation and Progression of Microbially Influenced Corrosion

Abstract

The subject of this report is the development of a screening method for detection of proteins in oil pipeline fluids and solids that could serve as indicators of microbially influenced corrosion (MIC). A method was developed that could detect proteins in sodium dodecyl sulfate (SDS) extracts of oil pipeline solids by SDS polyacrylamide gel electrophoresis (SDS-PAGE). Procedures for sample preparation, electrophoresis, and staining for visualization are described. Results showed that different protein banding patterns could be observed in samples obtained from different locations. A change in banding pattern was also observed in samples collected from one area, 3 and 7 months later.

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Document Details

Document Type
Technical Report
Publication Date
May 31, 1992
Accession Number
ADA253721

Entities

People

  • Victoria G. Prevatt

Tags

DTIC Thesaurus Topics

  • Acids
  • Alcohols
  • Biological Staining And Labeling
  • Buffers (Chemistry)
  • Corrosion
  • Detection
  • Electrophoresis
  • Extraction
  • Gel Electrophoresis
  • Identification
  • Indicator Dyes
  • Materials
  • Methanols
  • Molecular Weight
  • Pipelines
  • Proteins
  • Tubes

Fields of Study

  • Chemistry

Readers

  • Computer Vision.
  • Materials Science and Engineering.
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology