Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes,
Abstract
There are three main objectives of the research conducted under the above contract. First to isolate hematopoietic stem cells in numbers adequate for transfection with rearranged immunoglobulin genes. Second, to develop techniques which will allow B cells expressing the transfected Ig-gene product to be activated by anti-idiotypic antibodies so that high levels of serum antibody are produced. Finally, to develop a human-mouse chimera that will allow us to transfect rearranged Ig-genes into human hematopoietic progenitors then grow and activate those cells in an animal model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1992
- Accession Number
- ADA253974
Entities
People
- James J. Kenney