Construction and Evaluation of a Polyvalent Genetically Engineered Vaccine Candidate for VEE.
Abstract
We have shown previously that combining multiple independently attenuating mutations in a single Venezuelan equine encephalitis virus (VEE) strain using recombinant DNA techniques is a feasible strategy for producing a stable, immunogenic live virus vaccine. To improve the initial vaccine candidate, two major areas have been pursued. First, the number of attenuating loci from which the constituent mutations will be selected has -been expanded by further study of rapid penetration mutants, the investigational VEE vaccine strain, TC-83, and regions conserved among alphaviruses. Second, testing of alternate mutations at known attenuating loci has been initiated. Attenuating mutations have been compared in different strains of mice, and in a single strain infected by two different routes. These comparisons will aid in the selection of the two or three c t mutations of the improved vaccine candidate. A shuttle vector has been constructed to facilitate the combination of different glycoprotein mutations with mutations in other regions of the genome.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 11, 1992
- Accession Number
- ADA255244
Entities
People
- Nancy L. Davis
- Robert E. Johnston
Organizations
- University of North Carolina at Chapel Hill