The 3D Structure of Some Diarrheal Causing Bacterial Toxins

Abstract

The major objective of this research project, to determine the 3D crystal structure of the staphyloccal enterotoxin Beta (SEB), was attained. The structure was solved to a resolution of 2.5 A, and the final result has been submitted for publication to the scientific journal, Nature. The T cell receptor binding site was identified and was demonstrated in a sterically detailed manner with the aid of knowledge obtained independently in a mutational analysis. Regions implicated in the binding of SEB to MHC class II molecules also were elucidated in steric detail. All of the SEs (A to E inclusive) are predicted to s the SEB main chain fold. Staphylococcal enterotoxins; 3D structures; X-ray diffraction; Protein crystallography; Superantigen; RA 4.

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Document Details

Document Type
Technical Report
Publication Date
Jul 23, 1992
Accession Number
ADA255257

Entities

People

  • Martin Sax

Organizations

  • Veterans Administration Medical Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bacterial Toxins
  • Biological Toxins
  • Cells
  • Crystal Lattices
  • Crystal Structure
  • Crystallization
  • Crystallography
  • Crystals
  • Data Sets
  • Diffraction
  • Food Poisoning
  • Lymphocytes
  • Materials
  • Molecules
  • Three Dimensional
  • X Rays
  • X-Ray Diffraction

Fields of Study

  • Chemistry

Readers

  • Microbial Pathology
  • Molecular and Cellular Biochemistry
  • Technical Research and Report Writing.