The Synthesis of Chemiluminescent Flavoabzymes and Their Use in Metal Ion Detection.
Abstract
The ultimate goal of this research is to develop chemiluminescent (CL) abzymes and a technology for their use in metal ion detection. Four objectives must be reached in sequential order:.1st, a molecule (hapten) which resembles in space requirements an intermediate in a CL reaction must be synthesized; 2nd, a hapten protein conjugate (antigen) must be prepared and used to obtain monoclonal antibodies (Mab's); 3rd, one or more of the Mab's must catalyze the given CL reaction; and .4th , the hapten must be-modifiable such that the CL Mab's become specific complexers of metal ions. Accomplishment of the 1st objective would provide the first Mab mimic of bioluminescence. Attempts to synthesize a hapten to generate Cl flavoabzymes will be discussed as will progress in the synthesis of a hapten to be used to prepare abzymes to catalyze the CL reaction of H202 with oxalate esters Monoclonal antibodies (Mab's); Abzymes; hapten; antigen; chemiluminescence (CL); bioluminescence.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 02, 1992
- Accession Number
- ADA255567
Entities
People
- Thomas C. Bruice
Organizations
- University of California, Santa Barbara