Acetylcholinesterase and Acetylcholine Receptor.

Abstract

Our previous studies on cationic and uncharged substrates and reversible aliphatic and aromatic inhibitors led to the conclusions (1) that the subsite at which the trimethylammonio group of acetylcholine (AcCh) binds to acetylcholinesterase (AcChE) is not 'anionic' but uncharged 'trimethyl' in character; (2) contiguous to it are aromatic aminoacid sidechains; (3) the cationic and uncharged groups bind at the same site; and (4) uncharged reagents would more specifically characterize the active site. Bromopinacolone, (CH3) 3CCOCH2Br (BrPin) , Ki(com)= 0.2 mM, inactivated AcChE from Electrophorus with the same efficiency toward cationic and uncharged substrates. Acetylcholinesterase, Active Site, Label, Inhibitors, RA5.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1992
Accession Number
ADA255623

Entities

People

  • Saul G. Cohen

Organizations

  • Brandeis University

Tags

Communities of Interest

  • Biomedical
  • Weapons Technologies

DTIC Thesaurus Topics

  • Alcohols
  • Amines
  • Amino Acids
  • Chemical Synthesis
  • Chemistry
  • Ketones
  • Liquid Chromatography
  • Organic Chemistry

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Neurotoxicology
  • Polymer Science and Technology