Use of Protein A as the Primary Layer in Fluorescent Microsphere Technology.
Abstract
Six unique monoclonal antibodies (mAb) to the galactose-binding adherence lectin of Entamoeba histolytica were assessed for their abilities to bind protein A, using BIAcore. Of the six antibodies tested, mAb H85, specific for pathogenic strains, showed the greatest degree of binding to Protein A. Therefore, protein A was used as a surface layer on microspheres to which mAbs H85 and 3F4 were attached. Conversion of this method to microsphere agglutination and enzyme linked immunabsorbent assay (ELISA) formats proved successful. Instrumented reads of agglutination reactions in the ELISA format achieved a lower limit of detection equivalent to 500 amoeba when performed on solubilized amebic antigen. Protein A, Fluorescence, Epitope, Adherence lectin, Microsphere, Monoclonal antibody.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1992
- Accession Number
- ADA255631
Entities
People
- Rosalyn Slease
- Sheila J. Wood
- William A. Petri
Organizations
- Edgewood Chemical Biological Center