Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Plasmodium vivax-VK247 Sporozoites

Abstract

An enzymed-linked immunosorbent assay (ELISA) for the Plasmodium vivax-VK247 (variant) circumsporozoite (CS) protein was developed and evaluated using sporozoites produced by feeding mosquitoes on Thai patients with parasitologically confirmed P. vivax infections. The ELISA had a detection threshold of fewer than 50 sporozoites. Using this assay in conjunction with an ELISA for the VK210 polymorph, nearly 16% of the 235 P. vivax cases produced sporozoites positive only for the variant; 69% produced sporozoites positive only in the VK210 assay; and 15% were positive in both assays, indicating mixed infections. Twelve cases (5%) produced sporozoites negative-in one assay and with unexpectedly low activity in the other ELISA, indicating the possibility of other CS protein polymorphs. Plasmodium vivax, circumsporozoites protein detection ELISA.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1992
Accession Number
ADA256049

Entities

People

  • Jetsumon Sattabongkot
  • Robert A. Wirtz
  • Ronald Rosenberg
  • Ted Hall
  • Thomas R. Burkot

Organizations

  • Walter Reed Army Institute of Research

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Antibodies
  • Cells
  • Classification
  • Coinfection
  • Culture Techniques
  • Detection
  • Diseases And Disorders
  • Geographic Regions
  • Infection
  • Infectious Diseases
  • Proteins
  • Public Health
  • Recombinant Proteins
  • Security
  • Test And Evaluation
  • Wound Infections

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology and Pathology
  • Parasitology and Pharmacology of Malaria.