Purification and Partial Characterization of Monoclonal Antibodies of Francisella tularensis

Abstract

Hybridomas secreting monoclonal antibodies against Francisella tularensis cellular antigens were isolated. These monoclonal antibodies reacted with killed whole cells of F. tularensis in the ELISA but not by immunoblot, indicating that the antibodies were directed against conformational epitopes. One of these monoclonal antibodies was directed principally against outer membrane protein (OMP) components, and the remainder are likely directed against capsular components. The OMP-specific monoclonal antibodies are F. tularensis- specific in contrast to the others which cross-react with a number of other bacterial species. These OMP-specific monoclonal antibodies are of the IgG1 subclass, and may prove to be a useful tool for diagnosis of F. tularensis in clinical materials.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1992
Accession Number
ADA257092

Entities

People

  • A. R. Bhatti
  • Donald E. Woods
  • J. P. Wong

Organizations

  • Defence Research and Development Canada

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Antigens
  • Bacteria
  • Cell Line
  • Cells
  • Classification
  • Culture Techniques
  • Cultured Cells
  • Diseases And Disorders
  • Gammaproteobacteria
  • Gram-Negative Bacteria
  • Health Services
  • Infection
  • Infectious Diseases
  • Materials
  • Membrane Proteins
  • Microbiology

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry