Catalytic Antibodies for Prophylaxis/Treatment of Cyanide Poisoning
Abstract
The prophylactic administration of a drug capable of reacting quickly with cyanide and removing it from the circulation is one approach to the problem of cyanide intoxication. Alpha, Beta-Unsaturated ketones, esters, nitriles, etc. , undergo 1,4-addition with hydrogen cyanide to form stable compounds. We have investigated the reaction of cyanide with 4-phenyl-3-buten-2-one at 37 deg C and pH 7.4 in aqueous phosphate buffer. Disappearance of 4-phenyl-3-buten-2-one and appearance of the product 4-phenyl-4-cyanobutan-2-one were followed by HPLC analysis on a reverse phase column. The reaction rate was first order in enone and KCN with a rate constant of 0.024 to 0.082 M-lh-1. Catalysis of such reactions by an antibody to the transition state for cyanide addition offers a potential for enhancement of the rate to useful levels. The development of such antibodies by use of transition state analogs as haptens is our overall goal. The antibodies would be combined with a drug which is a substrate for cyanide addition. Design and synthesis of the potential drug and its transition state analog are being carried out.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 09, 1991
- Accession Number
- ADA257229
Entities
People
- C. E. Cook
Organizations
- RTI International