A Highly Specific Radioimmunoassay for the Measurement of Caffeine in Saliva,

Abstract

Using a tritiated (3H) caffeine tracer and a murine monoclonal anti- caffeine antibody, we developed a radioimmunoassay (RIA) for the detection of caffeine (1,3,7 trimethylxanthine) in saliva. The assay shows <2% cross reactivity with theophylline and avoids interference from anti-mouse immunoglobulin (IgG) constituents found in serum but not in saliva. Saliva caffeine represents the unbound (biologically active) fraction of the drug. Assay values correlate well (r=.44, p<.05) with oral caffeine doses between 150 and 400 mg. The half-life of salivary caffeine of about six hours, based on the elimination curve for the subjects who received 400 mg, agrees with previous measurements of its half-life in serum. Caffeine, Saliva, Radioimmunoassay, Half-life.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1992
Accession Number
ADA258365

Entities

People

  • Joseph Assmus
  • Paul Naitoh
  • Robert T. Rubin
  • Scott S. Mcgeoy
  • Tamsin L. Kelly

Organizations

  • Naval Health Research Center

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Biomedical Research
  • Blood
  • Caffeine
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Elimination
  • Health Services
  • Immunoassay
  • Immunoglobulins
  • Liquid Chromatography
  • Measurement
  • Medical Personnel
  • Radioimmunoassay
  • Reactivities
  • Therapy

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Molecular and Cellular Biochemistry
  • Urban Planning and Geography.