Dietary Refinements in a Sensitive Fish Liver Tumor Model
Abstract
A purified diet has been developed and proven adequate for growth of the medaka (oryzias latipes), a sensitive teleost species proven responsive to hepatocarcinogenic agents. The overall nutritional adequacy of the diet, a casein based ration, was evaluated and compared to three diets: commercially available flaked fish food; live newly hatched Artemia; and a combination of flake diet supplemented with Artemia. Survival, growth, reproductive success, general and liver histopathology, and selected hepatic enzyme activities were compared in medaka from first feeding through reproductive maturity. The PC-diet proved adequate in all of the above criteria. This diet provides a standardized, nutritionally adequate and consistent alternative to undefined conventional diets and is less likely to contain the range of xenobiotics possible in whole live food. Studies in progress indicate the suitability of the diet for aquatic toxicity/carcinogenicity studies. Teleost, Liver, Carcinogenesis, Medaka, Oryzias latipes, Model, Diet, RAIII, Fish.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 20, 1991
- Accession Number
- ADA258540
Entities
People
- David E. Hinton
Organizations
- University of California, Davis, School of Veterinary Medicine