Molecular Analysis of Medaka Tumors: New Models for Carcinogenicity Testing
Abstract
The broad, long-term objective of our laboratory is to use fish as a model system to better understand factors which promote tumor production and to develop a reliable and sensitive means of detecting carcinogens in aqueous environments. Studies of oncogene activation and tumor production in fish will contribute to our understanding of the molecular basis of carcinogenesis. Increased knowledge of molecular mechanisms of tumor progression in vivo may be used in the development of sensitive systems for the detection of potentially harmful environmental contaminants. In these studies we have examined the role of oncogene activation in tumors in Japanese medaka (Oryzias latipes) induced by specific carcinogens. The medaka has been widely used for carcinogenicity testing in the past decade. In studies described here, we used fish which were exposed to diethylnitrosamine (DEN) and methylazoxymethanol acetate (MAMAc). A significant proportion of exposed animals developed a variety of liver tumors. DNA extracted from these livers was analyzed in transfection assays for the presence of transforming genes. We have also initiated studies on oncogene expression in normal tissue and are continuing the analysis of the sequence of a novel oncogene detected in a DEN-induced cholangiocarcinoma.... Carcinogenesis, Oncogenes, Fish model, Aquatic toxicology, Lab animals, Environmental health, RAIII.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 06, 1992
- Accession Number
- ADA259005
Entities
People
- Rebecca J. Van Beneden
Organizations
- Duke University Hospital