Specific Detection of Campylobacter Jejuni and Campylobacter Coli by Using Polymerase Chain Reaction
Abstract
Development of a routine detection assay for Campylobacter jejuni and Campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction(PCR). An oligonucleotide primer pair from a conserved 5' region of the flaA gene of C. coli VC167 was used to amplify a 450-bp region by PCR. The primer pair specifically detected 4 strains of C.coli and 47 strains C. jejuni; but it did not detect strains of Campylobacter fetus, Campylobacter lari, Campylobacter upsaliensis, Campylobacter cryaerophila, Campylobacter butzleri, Campylobacter hyointestinalis, Wolinella recta, Helicobacter pylori, Escherichia coli, Shigella spp., Salmonella spp., Bibrio cholerae, Citrobacter freundii, or Aeromonas spp. By using a non radioactively labeled probe internal to the PCR product, the assay could detect as little as 0.0062 pg of purified C. coli in DNA, or the equivalent of four bacteria. In stools steeded with C. coli cells, the probe could detect between 30 and 60 bacteria per PCR assay. the assay was also successfully used to detect C. coli in rectal swab specimens form experimentally infected rabbits and C.jejuni in human stool samples.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1992
- Accession Number
- ADA259161
Entities
People
- Buhari A. Oyofo
- Donald H. Burr
- Olgerts R. Pavlovskis
- Scott A. Thornton
- Trevor J. Trust
Organizations
- Naval Medical Research Center