Novel System for Testing Dermal and Epidermal Toxicity in Vitro
Abstract
The purpose of this Phase I project has been the modification and characterization of a dermal equivalent and full-thickness skin substrate as models for cytotoxicity and penetration studies. The dermal equivalent consists of mitotically and metabolically active fibroblasts and naturally secreted matrix proteins. A dermal/epidermal junction is present in the full-thickness skin model along with stratified keratinocytes and a stratum corneum. The work has involved optimization of cell growth and uniform preparation of the three- dimensional (3D) substrates, establishment of 'standard operating procedures to ensure quality control and reproducibility of test material, adaptation of commonly utilized in vitro cytotoxicity assays for use with our substrates, performance of standard tests using a series of known toxicant and irritants in conjunction with the 3D substrates, and correlation of in vitro data with known in vivo toxicity and irritancy values.... In vitro human skin model, Human cells, Dermal toxicity, Screening tests, RA III, SBIR.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 15, 1990
- Accession Number
- ADA259321
Entities
People
- Gail K. Naughton