Electrode Probes for the Rapid Assay of Seafood Toxicants. Phase 1

Abstract

An enzyme-limited immunosorbent-assays (ELISA) with electrochemical detection was developed for the seafood toxin okadaic acid (diarrhetic shellfish poison). At low ppb concentrations, competitive heterogeneous immunoassays were performed in which soluble sample antigen (50 pl) competed with immobilized antigen for soluble antibody (50 pl). Excess sample and antibody were removed by washing and a secondary antibody labeled with alkaline phosphatase was added. The enzyme label hydrolyzed phenyl phosphate to yield phenol which was oxidized at +870 mV vs. Ag/AgCl. The dynamic response was inversely proportional to soluble antigen concentration.... Electrochemical immunoassay, seafood toxins, okadaic acid, saxitoxin.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1990
Accession Number
ADA259402

Entities

People

  • G. G. Guilbault
  • G. J. Lubrano
  • Graham Ramsay
  • L. L. Nordyke

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Biosensors
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Detection
  • Detectors
  • Dynamic Response
  • Electrodes
  • High Pressure
  • Immunoassay
  • Immunosensors
  • Liquid Chromatography
  • Medical Personnel
  • Shellfish
  • Water

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Microbial Pathology