A Medical Research and Evaluation (MREF) Facility and Studies Supporting the Medical Chemical Defense Program
Abstract
The purpose of this task was to produce and characterize rabbit anti- atropine antisera for future analytical studies, and to establish and validate the Walter Reed Army Institute of Research (WRAIR) atropine radioimmunoassay (RIA) at Battelle's Columbus Laboratories. For Phase I of this task, an atropine- BSA conjugate was prepared and characterized. This conjugate had an atropine:BSA molar ratio of approximately 32:1, and was used to immunize 12 atropinase-free New Zealand White rabbits for periods of 13 or 21 months using two separate schedules. Antisera were collected and titrated using the RIA procedure. Two rabbits having the highest titer were identified, and their sera were pooled for RIA development. Although the titers of these sera were 10-20- fold lower than antisera from Dr. Smallridge at WRAIR, the RIA characteristics were similar. Using a 1:600 dilution, standard curves were generated with consistent detection limits of 3.0 ng/ml and response ranges extending to 20 ng/ml. A representative standard curve had a slope of 0.984, a y-intercept of 5. 975, an ED,, of 432.7 pg/50 microliters, and a bound cpm/total cpm (B/T) value of 0.32.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1992
- Accession Number
- ADA259484
Entities
People
- Balwant S. Bhullar
- David W. Hobson
- Larry S. Miller
- Victor S. Moore
Organizations
- Battelle Memorial Institute