Simultaneous Detection of Multiple Disease States.
Abstract
The objectives for this project were to demonstrate the utility of BioStar Optical ImmunoAssay (OIA) tm for the detection of infectious agents in insect populations using malaria and Lyme antigens as models. Also to demonstrate 'DIA applicability for the diagnosis of host response to malarial infections. To meet the latter objective a recombinant antigen specific to P. falciparum was immobilized to a propertiary solid support, which provided the desired optical properties, and samples assayed for the presence of antibody to this antigen. Specificity of this antibody response was clearly demonstrated. For the detection of Malaria or Lyme antigens, the appropriate monoclonal antibodies were immobilized to the optical solid support. Samples were examined for the presence of antigen in heat-inactivated organisms or infected carriers (mosquitos or ticks). Developed extraction protocols allowed uninfected organisms to be assayed without the generation of a signal. OIA's flexibility allows any number of analytes to be assayed in a panel forma And due to its simplicity, OIA has been demonstrated to be generally applicable to a wide range of testing environments. Since unstable reagents are not generally employed there are no special storage requirements for field tests.... Interference signal, Immunoassay.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 14, 1990
- Accession Number
- ADA259792
Entities
People
- Luis Lopez