Detection of Picogram Quantities of Botulinus Toxin-B Using the Light Addressable Potentiometric Sensor.
Abstract
Detection of picogram quantities of Botulinus toxin-B (BoTX), using Protein A purified biotin- and fluorescein-labelled antibodies, is described in this report. All assays were performed on the Light Addressable Potentiometric Sensor. Results indicate a near linear dose response curve for increasing the amounts of BoTX (10-1,000 pg). The presence of 1-30 ng of toxin resulted in a dose response curve that yielded high output signals (>4,000 microV/s) at saturation (approximately 25 ng). When challenged with nine different venoms, the biosensor indicated very low output signals (15% control), which indicated minimal biosensor nonspecificity.... Receptors, Biosensors, Toxins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1992
- Accession Number
- ADA259908
Entities
People
- James J. Valdes
- James P. Chambers
Organizations
- University of Texas at San Antonio