Development of Dengue Vaccine, Phase 1
Abstract
Dengue virus is a mosquito borne, positive strand RNA virus responsible for hundreds of thousands of illnesses annually. No effective vaccine exists for any of the four dengue serotypes. The ultimate goal of our research is the production of a vaccine for dengue type 2. As a preliminary step toward this goal, we have cloned randomly primed cDNA fragments of dengue type 2 in the bacterial expression vector, lambda gtll. When cloned in the proper translational orientation and reading frame, the recombinant clones will express Beta-galactosidase-DEN2 fusion proteins. Recombinants expressing such fusion proteins can be detected immunologically. As judged by the frequency of interruption of the Beta-galactosidase gene, approximately 25% of the vectors contain inserts.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1987
- Accession Number
- ADA260281
Entities
People
- John M. Ivy