Determination of 5-Bromo-2'-Deoxyuridine (BrdU) in Well Water by High Performance Liquid Chromatography (HPLC)

Abstract

A liquid chromatographic method has been developed for the analysis of 5-Br deoxyuridine (BrdU) in well water. The samples are injected directly onto a high performance liquid chromatograph (HPLC) without further sample preparation. Separation of BrdU from possible interferences found in well water was achieved by using a C-18 column and a mobile phase containing water and methanol. A programmable ultraviolet (UV) detector was used to monitor the effluent for BrdU. HPLC provides a rapid and reproducible method for the analysis of BrdU. High Performance Liquid Chromatograph (HPLC), 5-Bromo- 2'deoxyuridine (BrdU), Ultraviolet (UV).

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1992
Accession Number
ADA260486

Entities

People

  • Alan B. Rosencrance
  • William E. Dennis

Organizations

  • United States Army Biological Warfare Laboratories

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Biomedical Research
  • Chromatographs
  • Chromatography
  • Data Science
  • Detection
  • Detectors
  • Gas Chromatography
  • Information Science
  • Linear Regression Analysis
  • Liquid Chromatography
  • Liquids
  • Literature
  • Materials
  • Methanols
  • Regression Analysis
  • Standards

Fields of Study

  • Chemistry

Readers

  • Organic Chemistry