0.3 MM Diameter Flexible Amperometric Lactate Probe.

Abstract

A flexible lactate electrode was made of 400 plus or minus 100 7 micrometers diameter carbon fibers, epoxy embedded in a 0.3 mm diameter polyimide tubing. The electrode was modified by precipitating on it the relatively insoluble complex formed between 1100 kd partially N-ethylamine quaternized poly((vinylpyridine)Os(bipyridine)2Cl)Cl (POs-EA) and lactate oxidase. The steady-state lactate electrooxidation current, at 2mM lactate concentration and at 22 deg C, was 4OO nA. The 5O plus or minus 10 microA/sq cm current density and the 2OmA/sq cm,/M sensitivity decreased only by 5% upon increasing the partial pressure of oxygen from 0.0 to 0.2 atm. The electrode retains its sensitivity after dry storage at 4 deg C for 4 months in air but loses at 37 deg C half of its sensitivity in 7 hours through polymer desorption when operated at 0.4V (SCE).

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 26, 1993
Accession Number
ADA260826

Entities

People

  • Adam Heller
  • Dan L. Wang

Organizations

  • University of Texas at Austin

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Adsorption
  • Blood
  • Carbon Fibers
  • Chemical Engineering
  • Classification
  • Current Density
  • Electrodes
  • Elements
  • Engineering
  • Graphitic Materials
  • Measurement
  • Microelectrodes
  • Military Research
  • Monitoring
  • Partial Pressure
  • Security
  • Steady State

Fields of Study

  • Chemistry

Readers

  • Electrochemical Surface Science
  • Molecular and Cellular Biochemistry
  • Optical Fiber Sensing and Electromagnetic Propagation.