Plasmid Stabilization to Insure Gene Expression
Abstract
All unstable (structurally) plasmid in Bacillus subtilis was stabilized by modifying the nutritional environment and reducing the size of the plasmid. The stabilization was explained solely by the effect of the growth rate ratio between cells containing modified and parental plasmids. The recombinant protein produced by the B. subtilis system was Beta-galactosidase from Escherichia coli: with the success achieved here, attention was shifted to cellulase production in Clostridium thermocellum including enzyme isolation and gene cloning.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 10, 1992
- Accession Number
- ADA261021
Entities
People
- Arnold L. Demain
Organizations
- Massachusetts Institute of Technology