Comparative Infectivity Determinations of Dengue Virus Vaccine Candidates in Rhesus Monkeys, Mosquitoes, and Cell Cultures

Abstract

Attenuation of virulent viruses are widely used for the development of viral vaccines through the years. These vaccines are made by serial passages of virus in cells from non-natural hosts. The first intents for the development of Dengue virus vaccines was mad by serial intracerebral passages of virus in infant of newly-weaned mice. Primary canine kidney (PCK) cell culture are also used for the attenuation of wild Dengue virus strains. Studies using these attenuated virus strains have been recently published. The objective of this contract was to determine the antibody response of rhesus macaques inoculated with different attenuated dengue virus preparations. Vaccine immunogenecity was determined by the plaque reduction neutralization test (PRNT). The PRNT titer of each serum sample was determined as described elsewhere.

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Document Details

Document Type
Technical Report
Publication Date
Jan 28, 1993
Accession Number
ADA261892

Entities

People

  • Edmundo Kraiselburd

Organizations

  • University of Puerto Rico

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Animals
  • Antibodies
  • Biological Sciences
  • Biomedical Research
  • Cells
  • Culture Techniques
  • Inhibition
  • Inoculation
  • Laboratory Animals
  • Materials
  • Monkeys
  • Neutralization
  • Primates
  • Puerto Rico
  • Rhesus Monkeys
  • Vaccination
  • Vaccines

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology