Antigenicity of Campylobacter Jejuni Flagella
Abstract
We studied the antigenicity of a wild-type flagellate and motile F(+) M(+) Campylobacter jejuni strain (81116) and two daughter mutants, one flagellate and immotile F (+)M(-) and one aflagellate and immotile F(-)M(-). By solium dodecyl sulfate-polyacrylamide gel electrophoresis of acid-extracted surface proteins, a 63-kilodalton (kDa) band identified from sheared flagella as the flagellar protein was present in the F(+)M(+) and F(+)M(-) strains but not in the F(-)M(-) strain. No other differences in protein profile among the three strains were noted. By Western blotting, serum from rabbits immunized with either the F(+)M(+) or F(-)M(-) strain detected a 63-kDa protein in the F(+)M(+) and F(+)M(-) strains but not in the F(-)M(-) strain. That the F(-)M(-) antiserum recognized the 63-kDa band suggests that small amounts of this protein or a cross-reacting antigen is present on the F(-)M(-) strain. By counterimmunoelectrophoresis of the acid-extracted preparations with immune sera, all three strains were found to share three major antigens, but a fourth antigen with a net positive charge was present only in the F(+)M(+) and F(+)M(-) strains. Antisera to five C. jejuni and two Campylobacter fetus strains recognized the 63-kDa protein oif purified F(+)M(+) flagella in Western blots, demonstrating that a common antigen is present, but enzyme-linked immunosorbent assay results suggest that the sharing of this antigen among Campylobacter strains is variable
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 28, 1986
- Accession Number
- ADA265460
Entities
People
- Diane G. Newell
- Guillermo I. Perez-perez
- Henry J. Cody
- Janet A. Hopkins
- Martin J. Blaser
Organizations
- Veterans Administration Medical Center