Rapid Detection of Mycobacteria in Patients with HIV Infection
Abstract
The Polymerase Chain Reaction (PCR) along with hybridization to chemiluminescent DNA probes was used to detect mycobacteria potentially present in patient specimens from the Mycobacteriology laboratory at Walter Reed Army Medical Center(WRAMC). DNA from the specimens were prepared by two different methods, and used in the PCR amplifications. of the specimens tested 88% were known to contain Mycobacteria. Of these, we were able to detect 20 (54%), though we were able to detec 82% (9 of 11) using a new genus specific primer set. With our M. tuberculosis primers, we were able to detect 21 of 28 samples that were positive by culture results (75%). We are continuing to evaluate these primer sets, as well as trying to design a primer set for M. avium.... RA 1, Mycobacteria, Mycobacterium avium, Mycobacterium tuberculosis, Polymerase Chain Reaction, DNA Probes, Rapid identification.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 10, 1993
- Accession Number
- ADA265567
Entities
People
- David C. Fritzinger
Organizations
- Armed Forces Institute of Pathology