Detection of Pathogenic Campylobacter Species in Blood Culture Systems
Abstract
Because differences in recognition of Campylobacter fetus and Campylobacter jejuni in systemic infectious may be due partially to differences in the ability to cultivate these organisms, we studied their growth characteristics in two widely used blood culture systems. In the Roche Septi- chek system over a broad range of inocula all strains were detected in broth within 2 days and on paddles within 3 days. In the BACTEC 6 B aerobic bottles C. jejuni and C. fetus took a median of 5 and 3 days, respectively, to reach a median of 2 days to reach the growth index threshold. However, in the BACTEC 7 D anaerobic bottles, C. fetus required a median of 2 days to reach growth index threshold, whereas for C. jejuni the median was greater than 10 days. The poor performance of C. jejuni in both BACTEC systems may have been due to unfavorable incubation atmospheres and may partially explain why C. jejuni bacteremia is so frequently detected. Overall, the Roche Septi-Chek system was excellent for detecting Campylobacter strains in blood cultures. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1986
- Accession Number
- ADA265655
Entities
People
- Martin J. Blaser
- Wen-lang Wang
Organizations
- Veterans Administration Medical Center