Regulation of TCR Signaling by CD45 Lacking Transmembrane and Extracellular Domains
Abstract
The CD45 protein is a transmembrane tyrosine phosphatase that is required for normal cell receptor (TCR)-mediated signaling. A chimeric complementary DNA encoding the intracellular enzymatically active portion of murine CD45 preceded by a short amino-terminal sequence from p60 c-src was transfected into CD45 minusT cells. Expression of this chimeric protein corrected most of the TCR signaling abnormalities observed in the absence of CD45, including TCR-mediated enhancement of tyrosine kinase activity and Ca 2+ flux. Thus, the enzymatically active intracellular portion of CD45 is sufficient to allow TCR transmembrane signaling
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1993
- Accession Number
- ADA265837
Entities
People
- Allan M. Weissman
- Barbara B. Niklinska
- Carl H. June
- Christopher M. Burns
- Sinisa Volarevic
Organizations
- Naval Medical Research Center