Neuropathological Consequences of Exposure to Sublethal Doses of Cyanide
Abstract
Experiments focused on the effects of cyanide on brain energy metabolism, microdialysis studies (to measure acid products of anaerobic metabolism, brain monoamines and metabolites, redox-active substances and amino acids), edema and histology. Wistar rats were infused iv with sodium cyanide (NaCN) until the animal lost its righting reflex; when righting returned, NaCN infusion was started again. The local cerebral glucose use (LCGU) was measured by the (14C)-2-deoxyglucose (2-DG) procedure. A shift from aerobic to anaerobic metabolism and the oxidation-reduction state of the brain extracellular fluid (ECF) were determined by intracerebral microdialysis. Immediately after iv infusion of NaCN, there is a robust increase in glucose use. Also, there is a dramatic increase, up to 5-fold, in lactate in the ECF indicating an increase in anaerobic metabolism. However, at 60 minutes after NaCN, LCGU is markedly reduced in all 45 brain regions measured, except for choroid plexus. Thus, the expected increase in glucose use as a result of anaerobic metabolism is brief. The marked increase in acid metabolites of dopamine and serotonin suggest that dopamine and serotonin may participate in the reduction.... Cyanide, Local cerebral glucose use, Brain edema, Intracerebral microdialysis, Lactate, Ascorbate, Potassium, Hydrogen Peroxide, Monoamines, Amino acids, RAV
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1992
- Accession Number
- ADA266241
Entities
People
- Fred E. Samson
- Stanley R. Nelson
- Thomas L. Pazdernik
Organizations
- University of Kansas Medical Center