Expression Cloning of the High Affinity Choline Transporter
Abstract
The goal of the intended research was to clone the cDNA for a high affinity choline transport protein and then study its properties. We used several strategies without success in isolating the choline transporter cDNA. Instead, we isolated several other transporter cDNAs and characterized in detail one of them, a cDNA coding for a GABA transporter that is localized to glial cells in the cholinergic electromotor nucleus of Torpedo. In addition to these efforts, we cloned the cDNA for a novel modulator protein for presynaptic calcium channels. We are further characterizing this protein and the other polypeptides with which it interacts under the aegis of a new ARO contract.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 05, 1993
- Accession Number
- ADA266773
Entities
People
- Cameron B. Gundersen
Organizations
- University of California, Los Angeles