Solid-Phase Antibody Capture Hemadsorption Assay for Detection of Hepatitis A Virus Immunoglobulin M Antibodies
Abstract
A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from the test sera. HAV-specific IgM antibody is detected by the addition of HAV hemagglutinating antigen and goose erythrocytes. Hemadsorption of erythrocytes to antigen-antibody complexes attached to the solid phase indicate the presence of tgM antibodies. The SPACH assay was compared to a commercial radioimmunoassay and was found to be equally or more sensitive and specific for the detection of HAV IgM antibodies. The SPACH assay is an alternative, rapid assay that does not require hazardous substrates or radioactivity for the detection of HAV-specific antibodies....Hepatitis A, Virus, Antibodies
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 25, 1993
- Accession Number
- ADA267113
Entities
People
- D. R. Dubois
- L. N. Binn
- P. L. Summers
- P. W. Macarthy
- W. H. Cohen
Organizations
- Walter Reed Army Institute of Research