Genetic Variation of HIV: Viral Load and Genotypic Diversity in Relation to Viral Pathogenesis and Treatment
Abstract
Elucidation of Human Immunodeficiency Virus (HIV) pathogenesis and development of effective antiviral treatments and vaccines are priorities of the Army Retrovirus Program which require a thorough understanding of viral replication patterns, viral load (burden), and the molecular details of viral variation occurring in natural infection. Previously, little information existed concerning HIV-1 and HIV-2 replication and variation in vivo except for inferences drawn from studies of virus replication in cell culture; determinations of circulating p24 antigenemia, and genetic analysis of laboratory isolates of HIV. In our studies, we employed novel PCR (polymerase chain reaction), recombinant DNA, and virus culture approaches to study viral replication, burden, and variation in HIV infected individuals and in subjects receiving antiviral treatment. Specific aims were to: (1) Develop quantitative PCR-based approaches for measuring HIV-1 and HIV-2 DNA and RNA in blood and bone marrow mononuclear cells (PBMC/BMMC) and in plasma.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 20, 1993
- Accession Number
- ADA268902
Entities
People
- George M. Shaw
Organizations
- University of Alabama