A Comparative Study Regarding the Association of Alpha 2U Globulin with the Nephrotoxic Mechanism of Certain Petroleum-Based Air Force Fuels
Abstract
Adult male rats have a strain, dose, and time-dependent renal proximal tubular degeneration induced by certain hydrocarbon compounds. We used rat strain variation (Fisher 344 and NCI Black Reiter) and different hydrocarbon compounds (JP-4, JP-8, decalin and trimethylpentane) to investigate the hydrocarbon-induced nephrotoxic response. Histochemical and morphometric evaluation of NCI-Black Reiter rats exposed to decalin and JP-8 indicated that this strain undergoes an intermediate form of the hydrocarbon-induced nephrotoxicity when compared to the albino Fisher 344 strain. The intermediate nephrotoxic response of the NCI-Black Reiter rat was characterized by approximately a two-fold increase in the number of acid phosphatase reactive lysosomes in renal tubular cells. The NBR rats did not demonstrate an increase in the size of the individual lysosomes, however, a characteristic lysosomal aggregation pattern occurred in renal tubular cells following hydrocarbon exposure. Light and electron microscopic immunohistochemistry revealed increased levels of A2U reactive sites in the renal tubular cell of F344 male rats exposed to decalin, trimethylpentane, JP-4 and JP-8. The nephrotoxic effect of decalin, trimethylpentane, JP-4 and JP-8 appeared to be equivalent as judged by renal tubular lysosomal alterations and increased A2U immunoreactive sites. Most lysosomal proteins in either control or treated animals were not reactive with specific antibodies against A2U. The relative number of A2U reactive sites per unit area did riot increase as lysosomes enlarged or became angular in response to hydrocarbon exposure. Alpha 2U globulin, Hyaline droplet nephropathy, Rats, Petroleum fuels, JP-4, JP-8, Cytoskeleton, Immunohistochemistry, Lysosomes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 1993
- Accession Number
- ADA269881
Entities
People
- Thomas E. Eurell
Organizations
- University of Illinois Urbana–Champaign